The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.
AUTOR(ES)
Watanabe, K
RESUMO
The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted a ploypeptide of 518 amino acids with a calculated molecular weight of 58,251. The value is in good agreement with that obtained from gel filtration and SDS/PAGE of GSH I. The initiation codon 5 bp downstream of putative Shine-Dalgarno sequence was an unusual TTG, which encoded methionine. For transcription, two sets of consensus promoter signals(-10 and -35 regions) overlapping each other were identified. The terminator signal shows the favored stem-loop structure with an adequate free energy delta G = -22.80 kcal/mol.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=311453Documentos Relacionados
- Arabidopsis thaliana gamma-glutamylcysteine synthetase is structurally unrelated to mammalian, yeast, and Escherichia coli homologs.
- GSH1, which encodes gamma-glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation.
- On the active site thiol of gamma-glutamylcysteine synthetase: relationships to catalysis, inhibition, and regulation.
- gamma-Glutamylcysteine and thiosulfate are the major low-molecular-weight thiols in halobacteria.
- Excretion of cysteine and gamma-glutamylcysteine moieties in human and experimental animal gamma-glutamyl transpeptidase deficiency.