The oriC unwinding by dam methylation in Escherichia coli.
AUTOR(ES)
Yamaki, H
RESUMO
It has been shown that dam methylation is important in the regulation of initiation of DNA replication in E.coli. The question then arises as to whether dam methylation in the oriC region mediates any structural changes in DNA involved in the regulation of initiation of DNA replication. We demonstrate that the thermal melting temperature of the oriC region is lowered by adenine methylation at GATC sites. The regulation of initiation of DNA replication by dam methylation may be attributed to the ease of unwinding at GATC sites in oriC.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=336717Documentos Relacionados
- Periodic formation of the oriC complex of Escherichia coli.
- Importance of state of methylation of oriC GATC sites in initiation of DNA replication in Escherichia coli.
- Effect of dam methylation on the activity of the E. coli replication origin, oriC.
- Transcripts within the replication origin, oriC, of Escherichia coli.
- Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli.
