The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80
AUTOR(ES)
Tung, Kuei-Shu
FONTE
The National Academy of Sciences
RESUMO
In budding yeast, many mutants defective in meiotic recombination and chromosome synapsis undergo checkpoint-mediated arrest at the pachytene stage of meiotic prophase. We recovered the NDT80 gene in a screen for genes whose overexpression bypasses the pachytene checkpoint. Ndt80 is a meiosis-specific transcription factor that promotes expression of genes required for exit from pachytene and entry into meiosis I. Herein, we show that the Ndt80 protein accumulates and is extensively phosphorylated during meiosis in wild type but not in cells arrested at the pachytene checkpoint. Our results indicate that inhibition of Ndt80 activity is one mechanism used to achieve pachytene arrest.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=17316Documentos Relacionados
- Phosphorylation and Maximal Activity of Saccharomyces cerevisiae Meiosis-Specific Transcription Factor Ndt80 Is Dependent on Ime2
- NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae.
- Structure of the sporulation-specific transcription factor Ndt80 bound to DNA
- Meiosis-specific protein selectively associated with sex chromosomes of rat pachytene spermatocytes.
- NDT80 and the Meiotic Recombination Checkpoint Regulate Expression of Middle Sporulation-Specific Genes in Saccharomyces cerevisiae