The pathway of E. coli RNA polymerase-promoter complex formation as visualized by footprinting.

AUTOR(ES)

Hofer, B

RESUMO

The pathway of E. coli RNA polymerase-promoter complex formation was probed by characterization of low temperature intermediates at the major coat protein promoter of phage fd DMA. Three different complexes could be distinguished. One of them represents the active 'open' complex, the other two have to be regarded as 'closed'. The promoter contacts of the lower temperature complexes were totally comprised within the contacts of the higher temperature complexes. Increase in temperature led to extension of contacts into the downstream direction, while the upstream border of the complexes remained virtually unchanged. Only in the 'open' complex contacts were extended beyond the start site of transcription.

Documentos Relacionados

• The pathway of E. coli RNA polymerase-promoter complex formation as visualized by footprinting
• Kinetics of RNA polymerase-promoter complex formation: effects of nonspecific DNA-protein interactions.
• Fine structure of E. coli RNA polymerase-promoter interactions: α subunit binding to the UP element minor groove
• Reorganisation of an RNA polymerase–promoter DNA complex for DNA melting
• RNA Polymerase-Promoter Interactions: the Comings and Goings of RNA Polymerase