The Presence and Possible Role of Zinc in RNA Polymerase Obtained from Escherichia coli
AUTOR(ES)
Scrutton, M. C.
RESUMO
Highly purified preparations of the DNA-dependent RNA polymerase obtained from Escherichia coli contain about 2 g-atoms of tightly bound zinc per mol (molecular weight 370,000) of enzyme. When the purified enzyme is fractionated on Sephadex G-150 or G-200, correlation is observed between the zinc and enzymic activity. Although some of the preparations examined also contain iron, copper, and magnesium, the content of these metal ions show no consistent correlation with RNA polymerase activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389452Documentos Relacionados
- Role of curved DNA in binding of Escherichia coli RNA polymerase to promoters.
- A Novel Form of RNA Polymerase from Escherichia coli
- Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase
- Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase
- A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.