The pseudorabies virus homology of the herpes simplex virus UL21 gene product is a capsid protein which is involved in capsid maturation.
AUTOR(ES)
de Wind, N
RESUMO
We mutagenized, mapped, and sequenced the pseudorabies virus (PRV) homology of gene UL21 of herpes simplex virus type 1. A polyclonal mouse antiserum against the protein encoded by the UL21 homolog was generated and used to monitor the expression and subcellular localization of the UL21-encoded protein. We found that the protein is identical to a previously detected PRV capsid protein. We analyzed viable PRV strains encoding mutant UL21 homologys, truncated by insertion of an oligonucleotide that contains stop codons in all reading frames. In two PRV mutants carrying the oligonucleotide at two sites within the gene, processing of newly replicated viral DNA was impaired. In addition, we show that one of the UL21 mutants has strongly reduced virulence for mice.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=240387Documentos Relacionados
- Pseudorabies virus protein homologous to herpes simplex virus type 1 ICP18.5 is necessary for capsid maturation.
- The UL21 gene products of herpes simplex virus 1 are dispensable for growth in cultured cells.
- Pseudorabies Virus UL37 Gene Product Is Involved in Secondary Envelopment
- Complex Formation between the UL16 and UL21 Tegument Proteins of Pseudorabies Virus
- The product of the UL12.5 gene of herpes simplex virus type 1 is a capsid-associated nuclease.