The rare transcripts of interrupted rRNA genes in Drosophila melanogaster are processed or degraded during synthesis.
AUTOR(ES)
Jamrich, M
RESUMO
About 50% of the ribosomal transcription units in females of the bobbed 8 mutant of Drosophila melanogaster contain an intervening sequence of 5 kb in the 28S region of the gene. We analysed the transcription of ribosomal genes in this mutant using electron microscopy and found that the majority of the active ribosomal transcription units in larval fat bodies and guts are not long enough to contain the 5-kb intervening sequence; only approximately 1% of active transcription units have a length consistent with the presence of the 5-kb intervening sequence. Transcription units of this length show an interrupted gradient of nascent RNA fibril lengths indicative of processing or degradation during transcription. The position of the discontinuity in RNA length coincides with the position of the intervening sequence. This observation suggests that even though RNA polymerase may infrequently transcribe an entire interrupted gene, the process does not result in a full-length RNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=557556Documentos Relacionados
- Unbalanced rRNA gene dosage and its effects on rRNA and ribosomal-protein synthesis.
- Transcription of Endogenous and Exogenous R2 Elements in the rRNA Gene Locus of Drosophila melanogaster
- Coordinated decreases in rRNA gene transcription factors and rRNA synthesis during muscle cell differentiation.
- Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.
- Conserved 5S rRNA complement to tRNA is not required for protein synthesis.
