The Relation of Size to the Relative Rates of Degradation of Intestinal Brush Border Proteins
AUTOR(ES)
Alpers, David H.
RESUMO
Proteins associated with intestinal brush borders and their various fractions were solubilized with sodium dodecyl sulfate and β-mercaptoethanol, and separated by electrophoresis on acrylamide gels containing sodium dodecyl sulfate. Brush borders contain at least 15 proteins or subunits, ranging in molecular weight from 19,000 to 270,000. The largest proteins (170-270,000 mol wt), including the disaccharidases, are removed from the brush borders by papain. Proteins belonging to the remaining membrane, including alkaline phosphatase, have an intermediate size (53-140,000 mol wt). The proteins corresponding to the filamentous “core” of the microvilli are the smallest (19-45,000).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=332961Documentos Relacionados
- Binding of diarrheagenic Escherichia coli to 32- to 33-kilodalton human intestinal brush border proteins.
- Binding of Yersinia enterocolitica to rabbit intestinal brush border membranes, mucus, and mucin.
- Intestinal brush border membranes contain regulatory subunits of adenylyl cyclase.
- Evolutionary matches of enzyme and transporter capacities to dietary substrate loads in the intestinal brush border
- Effect of Secreted Bacteroides Proteases on Human Intestinal Brush Border Hydrolases