The Rhodobacter capsulatus glnB gene is regulated by NtrC at tandem rpoN-independent promoters.
AUTOR(ES)
Foster-Hartnett, D
RESUMO
The protein encoded by glnB of Rhodobacter capsulatus is part of a nitrogen-sensing cascade which regulates the expression of nitrogen fixation genes (nif). The expression of glnB was studied by using lacZ fusions, primer extension analysis, and in vitro DNase I footprinting. Our results suggest that glnB is transcribed from two promoters, one of which requires the R. capsulatus ntrC gene but is rpoN independent. Another promoter upstream of glnB is repressed by NtrC; purified R. capsulatus NtrC binds to sites that overlap this distal promoter region.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=196364Documentos Relacionados
- Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters.
- Urea Utilization in the Phototrophic Bacterium Rhodobacter capsulatus Is Regulated by the Transcriptional Activator NtrC
- Expression of glnA in Escherichia coli is regulated at tandem promoters.
- Expression of the putA gene encoding proline dehydrogenase from Rhodobacter capsulatus is independent of NtrC regulation but requires an Lrp-like activator protein.
- Promoters regulated by the glnG (ntrC) and nifA gene products share a heptameric consensus sequence in the -15 region.
