The scattered distribution of actin genes in the mouse and human genomes.
AUTOR(ES)
Soriano, P
RESUMO
A hamster actin cDNA probe was used to localize actin genes on the major components of mouse and human DNAs, namely on the four families of fragments forming the bulk of these genomes. Over 20 EcoRI fragments hybridizing the probe could be detected; a different subset of these fragments was found in each component. Since the fragment families forming the major components of the mouse and human DNAs derive from very long chromosomal segments, the isochores , the presence of actin genes on all components provides evidence for their dispersion in both genomes. In situ hybridization of 125I-labeled probe to metaphase chromosomes in the presence of dextran sulfate confirmed this dispersion by showing that the 29-30 actin gene sites so identified are distributed on almost all chromosomes. Moreover, some human actin genes could be mapped on specific chromosomal segments; in particular, one gene was localized on the long arm of the X chromosome. Finally, three different mouse actin genes were isolated from a recombinant DNA library and previously investigated interspersed repeated sequences were identified in the vicinity of these genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553090Documentos Relacionados
- The distribution of interspersed repeats is nonuniform and conserved in the mouse and human genomes.
- KEGG: Kyoto Encyclopedia of Genes and Genomes.
- Structure of endogenous murine leukemia virus DNA in mouse genomes.
- Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.
- Organization and stability of endogenous xenotropic murine leukemia virus proviral DNA in mouse genomes.