The secondary structure of oocyte and somatic 5S ribosomal RNAs of the fish Misgurnus fossilis L. from nuclease hydrolyses and chemical modification data.

AUTOR(ES)

Serenkova, T I

RESUMO

We have studied the accessibility of 5'- 32P labeled oocyte and somatic 5S rRNAs from the fish Misgurnus fossilis L. to S1, T1 and cobra venom nucleases and have found that the cleavage sites of 5S rRNAs closely related in primary structures differ in these molecules. The data of nuclease hydrolyses revealed the existence of two conformers corresponding to renatured and partially denatured somatic 5S rRNA and capable of mutual interconversions. The exposed cytosine residues were located in oocyte and somatic 5S rRNAs converted into uridine ones by sodium bisulfite treatment. The data have been used to construct the secondary structure models of somatic and oocyte 5S rRNAs by means of specially devised computer program. These models differ in their 5'-halves which contain all the nucleotide substitutions in the primary structure, all differences in location of the exposed cytosine residues, and finally, in the cleavage pattern by the nucleases used.

Documentos Relacionados

• The primary structure of oocyte and somatic 5S rRNAs from the loach Misgurnus fossilis.
• Oocyte and somatic 5S ribosomal RNA and 5S RNA encoding genes in Xenopus tropicalis.
• The primary structure of 5S ribosomal RNAs from Magnolia cobus and Magnolia stellata.
• Secondary structure of eukaryotic cytoplasmic 5S ribosomal RNA.
• Secondary structure and phylogeny of Staphylococcus and Micrococcus 5S rRNAs.