The single pseudouridine residue in Escherichia coli 16S RNA is located at position 516.

AUTOR(ES)

Bakin, A

RESUMO

The number and location of pseudouridine residues in Escherichia coli 16S ribosomal RNA has been determined by a combination of direct and indirect methods. Only one residue was found, at position 516. This site is at the 5'-end of one of the three most highly conserved long sequences of this RNA molecule. A number of experimental findings have strongly implicated this loop in the fidelity of codon recognition by A-site bound tRNA. By virtue of its location, we suggest that psi 516 may also play a role in maintaining the fidelity of protein synthesis.

Documentos Relacionados

• Base changes at position 792 of Escherichia coli 16S rRNA affect assembly of 70S ribosomes.
• Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis.
• Alternative conformations in Escherichia coli 16S ribosomal RNA.
• Primary sequence of the 16S ribosomal RNA of Escherichia coli.
• Mutagenesis at the mRNA decoding site in the 16S ribosomal RNA using the specialized ribosome system in Escherichia coli.