The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc
AUTOR(ES)
Chen, Ping
FONTE
American Society for Microbiology
RESUMO
Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans. DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutR), the prepromutacin structural gene (mutA), a modifying protein (mutM), an ABC transporter (mutT), and an immunity cluster (mutFEG). Transformations of a non-mutacin-producing strain, S. mutans UA159, and a mutacin I-producing strain, S. mutans UA140, with chromosomal DNA from S. mutans T8 with an aphIII marker inserted upstream of the mutacin II structural gene yielded transformants producing mutacin II and mutacins I and II, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=91190Documentos Relacionados
- Structure-Activity Study of the Lantibiotic Mutacin II from Streptococcus mutans T8 by a Gene Replacement Strategy
- Functional Analyses of the Promoters in the Lantibiotic Mutacin II Biosynthetic Locus in Streptococcus mutans
- Diacylglycerol Kinase Is Involved in Regulation of Expression of the Lantibiotic Mutacin II of Streptococcus mutans
- Isolation and biochemical characterization of a novel lantibiotic mutacin from Streptococcus mutans.
- Genetic and Biochemical Analysis of Mutacin 1140, a Lantibiotic from Streptococcus mutans