The speed limit for protein folding measured by triplet–triplet energy transfer
AUTOR(ES)
Bieri, Oliver
FONTE
The National Academy of Sciences
RESUMO
A direct measure of intramolecular chain diffusion is obtained by the determination of triplet–triplet energy-transfer rates between a donor and an acceptor chromophore attached at defined points on a polypeptide chain. Single exponential kinetics of contact formation are observed on the nanosecond time scale for polypeptides in which donor and acceptor are linked by repeating units of glycine and serine residues. The rates depend on the number of peptide bonds (N) separating donor and acceptor and show a maximum for the shortest peptides (N = 3) with a time constant (τ = 1/k) of 20 ns. This sets an upper limit for the speed of formation of the first side-chain contacts during protein folding.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=22254Documentos Relacionados
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