The structure of the covalent linkage between proteins and RNA in encephalomyocarditis virus.

AUTOR(ES)

Vartapetian, A B

RESUMO

Two protein, VPgA and VPgB, are covalently bound to the virion RNA of encephalomyocarditis (EMC) virus. Their molecular weightrs, as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate are 10,000 and 8,000, respectively. A study of nucleotide-peptides isolated from VPg-RNA compound has shown that VPgA is bound to the 5'-terminal nucleotide of RNA by a phosphodiester bond. The 5'-terminal nucleotide of RNA is uridylic acid. It is the hydroxy group of the Tyr residue of VPgA that is involved in the formation of the linkage with RNA. VPgB-RNA seems to be similar to VPgA-RNA both in the structure of the RNA-protein linkage and localization of VPgB on RNA.

Documentos Relacionados

• Polyamines in encephalomyocarditis virus.
• Protein-RNA interaction in encephalomyocarditis virus as revealed by UV light-induced covalent linkages.
• Virus-induced diabetes mellitus. V. Biological differences between the M variant and other strains of encephalomyocarditis virus.
• Isolation and Subunit Structure of Polycytidylate-Dependent RNA Polymerase of Encephalomyocarditis Virus
• Genomic structure and RNA polymerase activity in Leishmania virus.