The study of biological meaning of multinucleation induced by vincristine in cultured cells / Estudo do significado biológico da multinucleação induzida por vincristina em células em cultura

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The study of agents that interfere in the functionality of proteins related to cell cycle is important for the understanding of the transformation and cell death processes. Although ploidy alterations are presented in the majority of human tumors, their role in oncogenic process is not understood yet. The alteration on chromosomal number is the primary consequence of errors involving the mitotic spindle and kinetocore. Thus, drugs acting on the microtubules are considered as potentially aneugenic agents. The present work aimed to study the mechanism of multinucleated cells induction by action of antimicrotubule drug and biological meaning of these cells. The results showed that vincristine induced mitotic arrest of both BBnt and MDCK cells, with consequent entrance into interphasic-multinucleated status. Multinucleated cells did not present features of cell death by apoptosis; they were still viable and able to go further in cell-cycle progression. The presence of many structures suggested microtubule enucleation, centrosomes-like were detected on treated cells and could be responsible for the multi-spindle assembling that leads the multinucleated cells to abnormal divisions. Later on, when the multinucleated cells accumulated more abnormalities they were eliminated from the cell population by apoptosis.

ASSUNTO(S)

multinucleation cultura celular cell culture citoesqueleto vincristine vincristina multinucleação cytoskeleton apoptose apoptosis aneuploidy aneuploidia

ACESSO AO ARTIGO

Documentos Relacionados