The transactivating domain of the c-Jun proto-oncoprotein is required for cotransformation of rat embryo cells.

AUTOR(ES)

Alani, R

RESUMO

The nuclear phosphoprotein c-Jun, encoded by the proto-oncogene c-jun, is a major component of the AP-1 complex. A potent transcriptional regulator, c-jun is also able to transform normal rat embryo cells in cooperation with an activated c-Ha-ras gene. By deletion analysis, we identified the regions of c-Jun encoding transformation and transactivation functions. Our studies indicate that there is a direct correlation between the ability of the c-Jun protein to activate transcription and cotransform rat embryo cells. The regions involved in these functions include the conserved leucine zipper/DNA binding domain and an effector domain near its N terminus. This N-terminal region spans amino acids 61 to 146 of the c-Jun protein and is highly conserved among all Jun family members. These results support the hypothesis that c-Jun transforms cells by stimulating the expression of transformation-mediating genes.

Documentos Relacionados

• c-Fos Proto-Oncoprotein Is Degraded by the Proteasome Independently of Its Own Ubiquitinylation In Vivo
• The solution structure of the RING finger domain from the acute promyelocytic leukaemia proto-oncoprotein PML.
• Induction of the proto-oncogene c-jun by angiotensin II.
• The MT domain of the proto-oncoprotein MLL binds to CpG-containing DNA and discriminates against methylation
• Nucleotide sequence of rat c-jun protooncogene.