The Ume6 regulon coordinates metabolic and meiotic gene expression in yeast
AUTOR(ES)
Williams, Roy M.
FONTE
The National Academy of Sciences
RESUMO
The Ume6 transcription factor in yeast is known to both repress and activate expression of diverse genes during growth and meiotic development. To obtain a more complete profile of the functions regulated by this protein, microarray analysis was used to examine transcription in wild-type and ume6Δ diploids during vegetative growth in glucose and acetate. Two different genetic backgrounds (W303 and SK1) were examined to identify a core set of strain-independent Ume6-regulated genes. Among genes whose expression is controlled by Ume6 in both backgrounds, 82 contain homologies to the Ume6-binding site (URS1) and are expected to be directly regulated by Ume6. The vast majority of those whose functions are known participate in carbon/nitrogen metabolism and/or meiosis. Approximately half of the Ume6 direct targets are induced during meiosis, with most falling into the early meiotic expression class (cluster 4), and a smaller subset in the middle and later classes (clusters 5–7). Based on these data, we propose that Ume6 serves a unique role in diploid cells, coupling metabolic responses to nutritional cues with the initiation and progression of meiosis. Finally, expression patterns in the two genetic backgrounds suggest that SK1 is better adapted to respiration and W303 to fermentation, which may in part account for the more efficient and synchronous sporulation of SK1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=129690Documentos Relacionados
- The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression.
- Identification of the Sin3-Binding Site in Ume6 Defines a Two-Step Process for Conversion of Ume6 from a Transcriptional Repressor to an Activator in Yeast
- UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression.
- The yeast UME6 gene product is required for transcriptional repression mediated by the CAR1 URS1 repressor binding site.
- Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae.