The Use of Specific Antibodies to Demonstrate the Glycocalyx and Spatial Relationships of a K99-, F41- Enterotoxigenic Strain of Escherichia coli Colonizing the Ileum of Colostrum-deprived Calves
AUTOR(ES)
Chan, R.
RESUMO
Electron microscopy was used to study the interaction between the glycocalyx of enterotoxigenic Escherichia coli strain 210 (09:K30+;K99-;F41-:H-) and the glycocalyx of epithelial cells in then ileum of experimentally infected newborn colostrum-deprived calves. Fixation of tissues in anti-K30 antibody and ruthenium red was used to stabilize the bacterial glycocalyx so that the spatial relationship between the bacteria and the intestinal epithelial cells could be characterized.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1235910Documentos Relacionados
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