The yeast nuclear gene suv3 affecting mitochondrial post-transcriptional processes encodes a putative ATP-dependent RNA helicase.

AUTOR(ES)

Stepien, P P

RESUMO

Mitochondrial gene expression is controlled largely through the action of products of the nuclear genome. The yeast nuclear gene suv3 has been implicated in a variety of mitochondrial posttranscriptional processes and in translation and, thus, represents a key control element in nuclear-mitochondrial interactions. We have exploited a property of a mutant allele of suv3, SUV3-1, that causes, among other effects, a massive increase in the abundance of excised group I introns to clone the wild-type gene by a strategy of colony Northern hybridization. We have determined that the 84-kDa deduced protein product of the suv3 gene, which maps to chromosome XVI, has a typical mitochondrial targeting presequence and additional sequence motifs that suggest that it belongs to a family of ATP-dependent RNA helicases, enzymes whose importance in post-transcriptional and translational events has recently become apparent. We have identified the SUV3-1 mutation as a G----T transversion that creates a Val----Leu substitution in a 10-amino acid block that is highly conserved among ATP-dependent RNA helicases. We discuss some implications of this mutation on the effects of the SUV3-1 allele on mitochondrial RNA metabolism.

Documentos Relacionados

• The nuclear SUV3-1 mutation affects a variety of post-transcriptional processes in yeast mitochondria.
• SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis
• A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.
• The E1 protein of bovine papilloma virus 1 is an ATP-dependent DNA helicase.
• HSP78 encodes a yeast mitochondrial heat shock protein in the Clp family of ATP-dependent proteases.