Three-dimensional structural model of eubacterial 5S RNA that has functional implications.
AUTOR(ES)
Pieler, T
RESUMO
Escherichia coli 5S RNA and its specific protein complexes were hydrolyzed with the single-strand-specific nuclease S1. Based on the results, a tertiary structural model for E. coli 5S RNA is proposed in which ribosomal proteins E-L5, E-L18, and E-L25 influence the conformation of the RNA. This may be of significance for ribosomal function. Comparison of the proposed E. coli 5S RNA structure with those of 18 other prokaryotic 5S RNAs led to a generalized eubacterial 5S RNA tertiary structure in which the majority of the conserved nucleotides are in non-base-paired regions and several conserved "looped-out" adenines (in E. coli, adenines -52, -53, -57, -58, and -66) are implied to be important for protein recognition or interaction or both.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=346722Documentos Relacionados
- The 9S RNA precursor of Escherichia coli 5S RNA has three structural domains: implications for processing.
- Reproducing the three-dimensional structure of a tRNA molecule from structural constraints.
- A three-dimensional working model for a guide RNA from Trypanosoma brucei.
- Three-dimensional model of the potyviral genome-linked protein.
- Three-dimensional model of yeast RNA polymerase I determined by electron microscopy of two-dimensional crystals.