THYMINELESS INDUCTION OF BACTERIOPHAGE IN BACILLUS MEGATERIUM1
AUTOR(ES)
Maisch, W. F.
RESUMO
Maisch, W. F. (University of Illinois, Urbana), and J. T. Wachsman. Thymineless induction of bacteriophage in Bacillus megaterium. J. Bacteriol. 88:1388–1393. 1964.—Strain KM:T−(L) of Bacillus megaterium was obtained by lysogenizing a previously characterized thymine auxotroph of strain KM (KM:T−) with a phage suspension from B. megaterium 899a. When strain KM:T−(L) is deprived of thymidine in an otherwise sufficient medium, both thymineless death and phage induction occur. The presence of thymidine is required, however, for phage development. When cells are starved for 2 hr, intracellular phage and free phage may be detected approximately 30 and 40 min, respectively, after thymidine addition. Maximal free phage titers were found to vary between 3 × 108 and 5 × 109 per ml. This represents as much as a 50,000-fold increase in free phage titer over that found during thymidine starvation, and up to a 1,000-fold increase over that found during normal exponential growth.
ACESSO AO ARTIGO
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