Timing of Enzyme Synthesis During Outgrowth of Spores of Bacillus cereus II. Relationship Between Ordered Enzyme Synthesis and Deoxyribonucleic Acid Replication
AUTOR(ES)
Steinberg, William
RESUMO
Experiments were carried out to determine whether, during outgrowth of bacterial spores, deoxyribonucleic acid (DNA) replication provided the basis by which ordered transcription was controlled. During outgrowth, significant DNA synthesis does not occur until just prior to the onset of cell division. However, incorporation of radioactive DNA precursors into DNA is observed within 5 to 10 min after the initiation of germination. By employing a thymine-requiring auxotroph and 3H-bromodeoxyuridine, this incorporation appears to be a result of DNA replication and not repair synthesis. For the following reasons it was concluded that, during outgrowth, transcriptional processes were not ordered by DNA replication. (i) In a thymine auxotroph, thymine addition did not alter the periodicity of induced α-glucosidase and histidase synthesis during outgrowth. (ii) DNA synthesis was inhibited 80% by 5-fluoro-2′-deoxyuridine (FUdR), and, after a 5-min lag, completely by mitomycin C, but these inhibitors exerted a differential effect on induced histidase synthesis. Enzyme synthesis was insensitive to FUdR but was inhibited by mitomycin C, presumably as a result of cross-linking of the complementary DNA strands.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252043Documentos Relacionados
- Timing of Enzyme Synthesis During Outgrowth of Spores of Bacillus cereus I. Ordered Enzyme Synthesis
- Relationship Between Protein and Ribonucleic Acid Synthesis During Outgrowth of Spores of Bacillus cereus
- Ordered Synthesis of Proteins During Outgrowth of Spores of Bacillus cereus
- Nature of Deoxyribonucleic Acid Synthesis and Its Relationship to Protein Synthesis During Outgrowth of Bacillus cereus T
- Method for Restricting Incorporation of Radioactivity from 3H-Thymidine into Deoxyribonucleic Acid Only During Outgrowth of Spores of Bacillus cereus T