Tissue inhibitor of metalloproteinases and collagenase inhibitory activity in lung secretions from patients with chronic obstructive bronchitis: effect of corticosteroid treatment.
AUTOR(ES)
Burnett, D
RESUMO
Tissue inhibitor of metalloproteinases (TIMP) and collagenase inhibitory activity were measured in the sputum from nine subjects with chronic bronchitis before and five days after treatment with corticosteroid (oral prednisolone, 40 mg daily). The mean sputum TIMP concentrations for eight of the nine subjects increased from 3.1 (SD 0.87) micrograms/ml to 5.8 (1.9) micrograms/ml (2p less than 0.005). Similarly, the mean collagenase inhibitory activity in the sputum of eight of the nine subjects increased from 1.53 (1.1) U/ml to 2.69 (0.92) U/ml (2p less than 0.05). The TIMP concentrations in sputum exceeded the collagenase inhibitory activity, suggesting that a proportion of the TIMP was inactive. TIMP inactivity was not due to prior complexing with enzyme since the molecular weight of sputum TIMP (27,500) was similar to that described for the purified protein (28,000-28,500). Preliminary studies showed the presence of TIMP in bronchoalveolar lavage samples (range of six specimens 0.45 ng/ml-2.1 micrograms/ml, median 53 ng/ml). Collagenase inhibitory activity was detected in only two of these six lavage samples, suggesting that the TIMP was totally inactive in the other four samples. The significance of the metalloproteinase-inhibitor balance in the pathogenesis of chronic lung disease requires further study.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=460466Documentos Relacionados
- The secretory IgA system of lung secretions in chronic obstructive bronchitis: comparison of sputum with secretions obtained during fibreoptic bronchoscopy.
- The secretory IgA system of lung secretions in chronic obstructive bronchitis
- Local IgA production in patients with chronic bronchitis: effect of acute respiratory infection.
- Effect of lung resection for bronchial carcinoma on pulmonary function in patients with and without chronic obstructive bronchitis.
- Effect of aminophylline on brain tissue oxygenation in patients with chronic obstructive lung disease.