Tissue-specific expression of the rat albumin gene: genetic control of its extinction in microcell hybrids.
AUTOR(ES)
Petit, C
RESUMO
Numerous studies of cell hybrids have indicated that somatic cells produce negative regulators (extinguishers) that prevent the expression of functions foreign to their own differentiation. Here, we report genetic evidence of such control. In microcell hybrids between well-differentiated rat hepatoma cells and microcells of mouse fibroblast L cells, the extinction of albumin synthesis is directly related to the presence of a single specific chromosome of the mouse fibroblast parent. The expression of several other hepatic functions is not affected. Transfection of these hybrids with a recombinant plasmid, containing a tissue-specific control element of the upstream region of the rat albumin gene linked to coding sequences of the chloramphenicol acetyltransferase gene, reveals that extinction acts on or via this cis-control element.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=323338Documentos Relacionados
- Tissue-specific expression of the rat galanin gene.
- Tissue-specific expression is conferred by a sequence from the 5' end of the rat albumin gene.
- Tissue-specific expression of the rat beta-casein gene in transgenic mice.
- Tissue-specific expression and developmental regulation of the rat apolipoprotein B gene.
- Tissue-specific expression of the rat alpha 2u globulin gene family.
