Tn10-derived transposons active in Bacillus subtilis.

AUTOR(ES)

Petit, M A

RESUMO

Small derivatives of the Escherichia coli transposon Tn10, comprising IS10 ends and a chloramphenicol resistance gene, were introduced in Bacillus subtilis on a thermosensitive plasmid, pE194. In the presence of the Tn10 transposase gene fused to signals functional in B. subtilis, these derivatives transposed with a frequency of 10(-6) per element per generation. They had no highly preferred insertion site or region, as judged by restriction analysis of the chromosomal DNA, and generated auxotrophic and sporulation-deficient mutants with a frequency of about 1%. These results suggest that Tn10 derivatives might be a useful genetic tool in B. subtilis and possibly other gram-positive microorganisms.

Documentos Relacionados

• Bacteriophage Lambda as a Delivery Vector for Tn10-Derived Transposons in Xenorhabdus bovienii
• Chromosomal insertions of Tn917 in Bacillus subtilis.
• Mutations that affect the translation efficiency of Tn9-derived cat gene in Bacillus subtilis.
• Translational block to expression of the Escherichia coli Tn9-derived chloramphenicol-resistance gene in Bacillus subtilis.
• Active transport of manganese in isolated membrane vesicles of Bacillus subtilis.