Topological and modulated distribution of surface markers on endothelial cells.
AUTOR(ES)
Nakache, M
RESUMO
The mobility and distribution of angiotensin-converting enzyme (peptidyl-dipeptide hydrolase, EC 3.4.15.1) and a specific endothelial cell surface protein was assessed by fluorescein-conjugated monoclonal antibodies on bovine and murine endothelial cells grown on their extracellular matrix. The combination of data obtained from fluorescence recovery after photobleaching measurements and observations under epifluorescence and total internal reflection fluorescence reveals a restriction of these protein markers to the apical membrane of endothelial cell. This asymmetry is evident both when cells are grown at a sparse density or at confluence. When cells are brought into suspension, the fluorescein-conjugated antibody is found over the entire cell surface. The fluorescence disappears from the basal part of the cell when the cells are again spread on coverslips coated with a layer of extracellular matrix. Conversely, cells spread on glass coverslips without extracellular matrix do not show this restriction phenomenon. It is suggested that the extracellular matrix provides the signal to induce the restricted topology of membrane protein markers on endothelial cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=323409Documentos Relacionados
- Distribution of Ia-like molecules on the surface of normal and leukemic human cells.
- Envelope glycoprotein of avian hemangioma retrovirus induces a thrombogenic surface on human and bovine endothelial cells.
- Distribution of receptors for transferrin and low density lipoprotein on the surface of giant HeLa cells.
- Assembly of vesicular stomatitis virus: distribution of the glycoprotein on the surface of infected cells.
- Isolation and characterization of a cell surface albumin-binding protein from vascular endothelial cells.