Torsional stress induces left-handed helical stretches in DNA of natural base sequence: circular dichroism and antibody binding.

AUTOR(ES)

Di Capua, E

RESUMO

Above a threshold of torsional stress, the c.d. spectrum of covalently closed circular DNA of natural base sequence acquires a Z-like contribution and antibodies raised against Z-DNA are bound. Mapping of the antibody binding sites by electron microscopy reveals sites which correlate with stretches enriched in alternating purine-pyrimidine sequences and GC base pairs.

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