Trans-splicing in chloroplasts: the rps 12 loci of Nicotiana tabacum.
AUTOR(ES)
Hildebrand, M
RESUMO
The rps12 gene in tobacco chloroplasts consists of three exons that code for a polypeptide with homology to Escherichia coli ribosomal protein S12. C-terminal exons 2 and 3 of rps12 are located in the inverted repeat regions of the tobacco chloroplast genome. Exon 1 of rps12 is 29 kilobase pairs downstream of the nearest copy of exons 2 and 3 and 69 kilobase pairs away from the distal copy of exons 2 and 3. RNA gel blot hybridization analysis and primer extension sequencing of cDNA to rps12 encoding RNAs indicate that exon 1 and exons 2 and 3 are encoded on separate transcripts. Exon 1 and exons 2 and 3 are covalently ligated in the correct reading frame in rps12 mRNA. These results indicate that a bimolecular (trans-) splicing event occurs during the formation of mature rps12 mRNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=279550Documentos Relacionados
- A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts.
- In vitro trans-splicing in Saccharomyces cerevisiae.
- mRNA 5′-leader trans-splicing in the chordates
- Experimental evidence for RNA trans-splicing in mammalian cells.
- Detection of a possible trans-splicing intermediate in Trypanosoma brucei.