Transcription by Methanothermobacter thermautotrophicus RNA Polymerase In Vitro Releases Archaeal Transcription Factor B but Not TATA-Box Binding Protein from the Template DNA

AUTOR(ES)

Xie, Yunwei

FONTE

American Society for Microbiology

RESUMO

Transcription initiation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding protein (TBP), transcription factor B (TFB), and RNA polymerase (RNAP). The results reported establish the fate of Methanothermobacter thermautotrophicus TBP and TFB following transcription initiation by M. thermautotrophicus RNAP in vitro. TFB is released after initiation, during extension of the transcript from 4 to 24 nucleotides, but TBP remains bound to the template DNA. Regulation of archaeal transcription initiation by a repressor competition with TBP for TATA-box region binding must accommodate this observation.

Documentos Relacionados

• TATA-box DNA binding activity and subunit composition for RNA polymerase III transcription factor IIIB from Xenopus laevis.
• Yeast TATA-box transcription factor gene.
• The archaeal TFIIEα homologue facilitates transcription initiation by enhancing TATA-box recognition
• Core promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding.
• Novel upstream elements and the TATA-box region mediate preferential transcription from the uteroglobin promoter in endometrial cells.