Transcription of 70S RNA by DNA polymerases from mammalian RNA viruses.
AUTOR(ES)
Abrell, J W
RESUMO
DNA polymerases purified by the same procedure from four mammalian RNA viruses, simian sarcoma virus type 1, gibbon ape lymphoma virus, Mason-Pfizer monkey virus, and Rauscher murine leukemia virus are capable of transcribing heteropolymeric regions of viral 70S RNA without any other primer. In this reconstituted system the enzymes from simian sarcoma virus type 1, Mason-Pfizer monkey virus, and Rauscher murine leukemia virus transcribe viral 70S RNA almost as efficiently as the DNA polymerase from the avian myeloblastosis virus, but gibbon ape lymphoma virus DNA polymerase is approximately three-to fivefold less efficient. Although there is a substantial difference among the sizes of these DNA polymerases (160,000 daltons for the avian myeloblastosis virus enzyme, 110,000 daltons for the Mason-Pfizer monkey virus enzyme, and 70,000 daltons for the mammalian type C viral polymerases), the ability to transcribe viral 70S RNA is a characteristic common to these enzymes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355765Documentos Relacionados
- Structure of 50 to 70S RNA from Moloney sarcoma viruses.
- Rapid Purification of 70S RNA from Media of Cells Producing RNA Tumor Viruses
- Transcription of DNA from the 70S RNA of Rous Sarcoma Virus II. Structure of a 4S RNA Primer
- Transcription of DNA from the 70S RNA of Rous Sarcoma Virus I. Identification of a Specific 4S RNA Which Serves as Primer
- Sedimentation coefficients of RNA from 70S and 80S ribosomes.
