Transcriptional activity and factor binding are stimulated by separate and distinct sequences in the 5' flanking region of a mouse tRNAAsp gene.

AUTOR(ES)

Rooney, R J

RESUMO

The transcriptional properties of two cloned mouse tRNAAsp genes were examined in vitro. The tRNA(2Asp) gene displays a five fold greater transcriptional activity than the tRNA(1Asp) gene and a greater ability to form stable complexes with transcription factors. Transcription of a hybrid gene with swapped 5' flanking sequences and of 5' flanking region deletion mutants demonstrates that the differential transcription of the genes results from stimulatory sequences in the 5' flanking region of the tRNA(2Asp) gene. Distal sequences including those between positions -53 and -31 stimulate transcription but do not affect factor binding. Proximal sequences between positions -9 and -1 enhance factor binding. Thus, binding of transcription factors and later steps required for transcription can be modulated by separate and distinct 5' flanking sequence motifs in eukaryotic tRNA genes.

Documentos Relacionados

• Studies of defective interfering RNAs of Sindbis virus with and without tRNAAsp sequences at their 5' termini.
• Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene.
• Identification of a TFIIIA binding site on the 5' flanking region of the TFIIIA gene.
• The 5' flanking region of human epsilon-globin gene.
• Structure and evolution of a mouse tRNA gene cluster encoding tRNAAsp, tRNAGly and tRNAGlu and an unlinked, solitary gene encoding tRNAAsp.