Transcriptional regulation of immunoglobulin V genes.
AUTOR(ES)
Mather, E L
RESUMO
The relative transcriptional activity of rearranged and unrearranged (germline) VK genes in secreting plasmacytoma cells was assessed by two independent methods. Measurements of V sequence abundance by hybridization kinetic (Rot) analysis indicated that the steady state content of transcripts from a rearranged VK gene is at least 16,000-fold greater than that from an unrearranged VK gene. Direct measurements of transcriptional activity in isolated nuclei indicated that this difference is due, in large part to a difference in transcription rate. Since the primary sequences of V genes and their 5' flanking regions are not altered during rearrangement, these results suggest that VK gene transcription might be controlled by elements on the 3' side of the VK genes or at the CK locus, perhaps via an influence on chromatin structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327647Documentos Relacionados
- Transcriptional regulation of Bacillus subtilis citrate synthase genes.
- Immunoglobulin V region variants in hybridoma cells. II. Recombination between V genes.
- Transcriptional regulation of two cytotoxic T lymphocyte-specific serine protease genes.
- Somatic hypermutation of immunoglobulin and non-immunoglobulin genes.
- Sequence analyses of three immunoglobulin G anti-virus antibodies reveal their utilization of autoantibody-related immunoglobulin Vh genes, but not V lambda genes.
