Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene.

AUTOR(ES)

Feinbaum, R L

RESUMO

We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.

Documentos Relacionados

• Blue-Light Regulation of the Arabidopsis thaliana Cab1 Gene.
• Analysis of flavanone 3-hydroxylase in Arabidopsis seedlings. Coordinate regulation with chalcone synthase and chalcone isomerase.
• Sequence of the Arabidopsis thaliana 7SL RNA gene.
• A gene encoding the tryptophan synthase beta subunit of Arabidopsis thaliana.
• The 1-aminocyclopropane-1-carboxylate synthase gene family of Arabidopsis thaliana.