Transduction of host cellular sequences by a retroviral shuttle vector.
AUTOR(ES)
Jhappan, C
RESUMO
We studied the frequencies and types of excision events which can occur with a retroviral shuttle vector containing the simian virus 40 origin of DNA replication. Analysis of the recloned vector plasmids by size and restriction enzyme mapping indicated that most contain one long terminal repeat. By hybridizing the plasmids to a mouse genomic repetitive DNA probe, we also determined that approximately 1 to 3% contain transduced cellular DNA sequences.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=288950Documentos Relacionados
- Cloning and assessment of mycobacterial promoters by using a plasmid shuttle vector.
- Cloning and characterization of a Bacteroides conjugal tetracycline-erythromycin resistance element by using a shuttle cosmid vector.
- High spontaneous mutation frequency in shuttle vector sequences recovered from mammalian cellular DNA.
- Construction and Characterization of a Replication-Competent Retroviral Shuttle Vector Plasmid
- Expression of a molecularly cloned human c-src oncogene by using a replication-competent retroviral vector.