Transfection of DNA into adherent cells by DEAE-dextran/DMSO method increases drastically if the cells are removed from surface and treated in suspension.
AUTOR(ES)
Golub, E I
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318065Documentos Relacionados
- Efficient transfer of cloned DNA into human diploid cells: protoplast fusion in suspension.
- Enhancement of Chlamydia trachomatis infectious progeny by cultivation of HeLa 229 cells treated with DEAE-dextran and cycloheximide.
- DEAE-dextran enhances electroporation of mammalian cells.
- Efficient transfection of DNA by mixing cells in suspension with calcium phosphate.
- High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.