Transfer of chromosomal genes and plasmids in Bacillus thuringiensis.

AUTOR(ES)

Aronson, A I

RESUMO

A low frequency of chromosomal gene transfer from Bacillus thuringiensis to Bacillus cereus was detected by cell mating, with a tryptophan marker being the most frequently transferred gene among four that were tested. The process was resistant to DNase and was not mediated by cell filtrates. Among several B. thuringiensis subspecies tested, transfer was best with a derivative of B. thuringiensis subsp. kurstaki HD1, which lost several plasmids. All of the B. cereus recombinants contained at least one plasmid from the donor B. thuringiensis; frequently, it was a plasmid that encoded a protoxin gene. In matings with B. thuringiensis subsp. kurstaki HD1, a 29-megadalton plasmid that contained a ca. 2.5-kilobase region of homology with the chromosome was always transferred. No detectable transfer of chromosomal genes was found in B. thuringiensis subsp. kurstaki HD1 strains lacking this plasmid, suggesting that there may be chromosome mobilization.

Documentos Relacionados

• Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis.
• Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis.
• Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis.
• In vitro transcription of the cloned chromosomal crystal gene from Bacillus thuringiensis.
• Transduction in Bacillus thuringiensis.