Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: Regeneration of fertile plants
AUTOR(ES)
Dudits, Denes
RESUMO
Transfer of methotrexate and 5-methyltryptophan resistance from carrot (Daucus carota) to tobacco (Nicotiana tabacum) was achieved by fusion between leaf mesophyll protoplasts of tobacco and irradiated cell culture protoplasts of carrot. Some of the regenerated somatic hybrids exhibited normal tobacco morphology with coexpression and independent segregation of the transferred resistance markers. Chromosomal instability resulted in aneuploid somatic hybrids with significantly lower chromosome number than predicted by simple addition of parental chromosome number. The methotrexate resistance phenotype was correlated with the expression of carrot-specific dihydrofolate reductase as judged by isozyme and immunological characteristics of the enzyme. The genomic construct of these somatic hybrids made the transmission of the resistance character into the next sexual generation possible.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=299558Documentos Relacionados
- Monoclonal antibodies against rabies virus produced by somatic cell hybridization: detection of antigenic variants.
- Regeneration of Fertile Barley Plants from Mechanically Isolated Protoplasts of the Fertilized Egg Cell.
- In Situ Transfer of Antibiotic Resistance Genes from Transgenic (Transplastomic) Tobacco Plants to Bacteria
- Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
- On the Beginnings of Somatic Cell Hybridization: Boris Ephrussi and Chromosome Transplantation