Transfer of tra proteins into the recipient cell during bacterial conjugation mediated by plasmid ColIb-P9.
AUTOR(ES)
Rees, C E
RESUMO
Selective transfer of the two products of the ColIb primase gene, sog, from donor to recipient cell during conjugation was demonstrated by two independent methods. The transfer of these tra proteins was unidirectional and dependent on DNA transfer. The Sog polypeptides were localized to the cytoplasm of the donor cell, but they appeared to interact with other tra gene products located in the inner membrane. After cell mating, the transferred polypeptides were found to be in the cytoplasm of the recipient cell, and it is estimated that as many as 500 Sog polypeptides were transferred per round of conjugation. It is proposed that these proteins are transferred as a result of an interaction with the single-stranded DNA and that the transferred strand may be coated with Sog polypeptides.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=210029Documentos Relacionados
- The ssb gene of plasmid ColIb-P9.
- Direction of conjugative transfer of IncI1 plasmid ColIb-P9.
- Organization of the replication control region of plasmid ColIb-P9.
- Nucleotide sequence of the gene (ard) encoding the antirestriction protein of plasmid colIb-P9.
- Structural and functional features of cis-acting sequences in the basic replicon of plasmid ColIb-P9.
