Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates.
AUTOR(ES)
Ogden, R C
RESUMO
The primary sequences of all the tRNA precursors which contain intervening sequences and which accumulate in the Saccharomyces cerevisiae rnal mutant are presented. A combination of DNA and RNA sequence analysis has led to elucidation of the primary sequence of four hitherto uncharacterized precursors. The location of the intervening sequence has in all cases been unambiguously determined by analysis of the intermediates in the splicing reaction. Secondary structures based upon the tRNA cloverleaf are shown for all the tRNA precursors and discussed with respect to common recognition by the yeast splicing endonuclease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320467Documentos Relacionados
- Direction of chromosome rearrangements in Saccharomyces cerevisiae by use of his3 recombinational substrates.
- tRNA Nuclear Export in Saccharomyces cerevisiae: In Situ Hybridization Analysis
- Messenger RNA stability in Saccharomyces cerevisiae: the influence of translation and poly(A) tail length.
- An Escherichia coli tyrosine transfer RNA is a leucine-specific transfer RNA in the yeast Saccharomyces cerevisiae.
- Expression of the clustered mitochondrial tRNA genes in Saccharomyces cerevisiae: transcription and processing of transcripts.