TransformaÃÃo de Stenocarpella maydis com os genes marcadores GFP e DsRed e patogenicidade dos transformados em sementes de milho / Transformation of Stenocarpella maydis with genes markers GFP and DsRed and pathogenicity of transformed into maize seeds

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

One of the fungi which can associate to maize seeds is Stenocarpella maydis, causal agent of stalk and ear rot, which is responsible by considerable losses in producing regions of this crop in Brazil. From literature, there is little information on the mechanisms of infection and on the transmission dynamics of this fungus in relation to maize seeds. For this sort of investigation the transformation of the fungus by mean of molecular markers such as GFP and DsRed.has been an important tool. So, this work was carried out aiming the transformation of the fungus and assessing its behavior in maize seeds. For transformation, the protocol used provided a reasonable number of protoplasts, reaching a satisfactory concentration for the purpose of this study. The plasmid was successfully multiplied and the concentration of the antibiotic was adequate, as it is recommended for other cases. The protocol was therefore adjusted and proved to be efficacy as demonstrated by the fluorescence in the fluorescence microscope. The transformed isolates maintained their morphological characteristics when compared to the original isolates, and their infectivity in maize seeds was confirmed. By the PCR technique, it was possible to confirm the presence of genes in the transformed isolates. From the inoculation of seeds it was possible to confirm the pathogenicity of the transformed isolates as well as it was possible to observe their colonization in the infected seeds.

ASSUNTO(S)

transformaÃÃo genÃtica gfp gfp milho fitopatologia protoplastos genetic transformation stenocarpella maydis dsred protoplasts corn dsred stenocarpella maydis

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