Transformation in Escherichia coli: cryogenic preservation of competent cells.
AUTOR(ES)
Morrison, D A
RESUMO
Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=221863Documentos Relacionados
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