Transformation of members of the genus Haloarcula with shuttle vectors based on Halobacterium halobium and Haloferax volcanii plasmid replicons.
AUTOR(ES)
Cline, S W
RESUMO
We have stably transformed both Haloarcula vallismortis and Haloarcula hispanica with the halobacterium-Escherichia coli shuttle vectors pWL102 (based on the Haloferax volcanii pHV2 replicon) and pUBP2 (based on the Halobacterium halobium pHH1 replicon). Haloferax volcanii, Halobacterium halobium, and Haloarcula vailismortis are equally distant from one another and span the phylogenetic depth of the halophilic Archaea; thus, these vectors may be generally useful for the halophiles. Both Haloarcula vallismortis and Haloarcula hispanica exhibit previously unreported complex life cycles and are therefore significant as genetically approachable models of cellular differentiation within the Archaea.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206205Documentos Relacionados
- Shuttle vectors for the archaebacterium Halobacterium volcanii.
- Cloning of genes from members of the family Enterobacteriaceae with mini-Mu bacteriophage containing plasmid replicons.
- Transformation of Halobacterium halobium: development of vectors and investigation of gas vesicle synthesis.
- Transformation of the archaebacterium Halobacterium volcanii with genomic DNA.
- Effects of salt and temperature on plasmid topology in the halophilic archaeon Haloferax volcanii.