Transformation of Rat Liver Cells with Chicken Sarcoma Virus B77 and Murine Sarcoma Virus
AUTOR(ES)
Altaner, C.
RESUMO
Rat liver cells in vitro were transformed with chicken sarcoma virus B77, giving RL(B77) cells, and with murine sarcoma virus (Harvey), giving RL(MSV) cells. Rat liver cells transformed spontaneously in vitro were designated RL cells. In addition, the RL(MSV) cell line was adapted for growth in culture fluid containing 25 μg of 5-bromodeoxyuridine per ml. All cell lines were tumorigenic in 1-wk-old rats. The number of cells needed for induction of tumor growth was 1,000-fold higher in the case of RL(B77) cells in comparison with RL(MSV) cells and RL cells. No production of viral particles from any of the cell lines investigated was detected by plating concentrated supernatant fluid of the cultures on different secondary embryo cells with and without fusion by Sendai virus, by labeling with uridine-5-3H, or by assay for deoxyribonucleic acid polymerase activity. The viral genome was rescued by fusion of RL(B77) cells with chicken cells. Chicken sarcoma virus rescued from (RL(B77) cells differed in plating efficiency on duck cells from B77 virus rescued from transformed rat embryo cells. No virus was rescued after fusion of RL(MSV) and RL cells with mouse, rat, or chicken embryo cells. Infectious murine sarcoma virus can be induced by 5-bromodeoxyuridine from RL(MSV) cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355080Documentos Relacionados
- Characterization of c-lil, a chicken cellular sequence associated with a stock of B77 avian sarcoma virus.
- Structure of B77 sarcoma virus RNA: stabilization of RNA after packaging.
- Evidence of methylation of B77 avian sarcoma virus genome RNA subunits.
- Particles of Reduced Infectivity and Deficient in Envelope Glycoproteins Are Produced in Cycloleucine-Treated B77 Avian Sarcoma Virus-Infected Chicken Embryo Fibroblasts
- Evidence for the Identity of Shared 5′-Terminal Sequences Between Genome RNA and Subgenomic mRNA's of B77 Avian Sarcoma Virus