Translation of Brome Mosaic Viral Ribonucleic Acid in a Cell-Free System Derived from Wheat Embryo
AUTOR(ES)
Shih, D. S.
RESUMO
The four RNAs of brome mosaic virus induce substantial incorporation of amino acids into protein when used as messengers in a cell-free protein-synthesizing system derived from wheat embryo. RNA 4 is highly efficient as a monocistronic messenger for the viral coat protein. Acetate, derived from acetyl coenzyme A, is incorporated into the product made in vitro. Although RNA 3 also contains the coat-protein cistron, it induces synthesis mostly of a protein larger than coat protein. RNAs 1 and 2 also induce the synthesis of substantial amounts of protein other than coat protein. However, an equimolar mixture of RNAs 3 and 4 or of 1, 2, 3, and 4 induces synthesis of coat protein almost exclusively. This result suggests that the coat-protein cistron, when present as a monocistronic messenger, inhibits translation of all other viral messages.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433600Documentos Relacionados
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