Translational repression of brlA expression prevents premature development in Aspergillus.
AUTOR(ES)
Han, S
RESUMO
The Aspergillus nidulans brlA developmental regulatory locus consists of two overlapping transcription units, brlA alpha and brlA beta, which encode functionally related polypeptides. We used translational fusions between each of the predicted brlA reading frames and the Escherichia coli lacZ gene to test the hypothesis that developmental regulation of brlA alpha and brlA beta expression occurs through different mechanisms. brlA alpha is transcriptionally controlled and a large portion of brlA alpha-directed beta-galactosidase activity is regulated in a brlA-dependent manner. In contrast, brlA beta mRNA is constitutively transcribed but translation of the brlA polypeptide is prevented by the presence of a short open reading frame (microORF) present in the 5' end of brlA beta mRNA. Removing the microORF initiation codon leads to deregulated brlA expression, resulting in an inappropriate activation of development. We propose that one mechanism for developmental induction in A.nidulans involves translational control.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=413480Documentos Relacionados
- FluG and flbA function interdependently to initiate conidiophore development in Aspergillus nidulans through brlA beta activation.
- brlA requires both zinc fingers to induce development.
- Developmental repression of growth and gene expression in Aspergillus.
- The Aspergillus nidulans brlA regulatory locus consists of overlapping transcription units that are individually required for conidiophore development.
- Kinetic studies on the specificity of chelate-iron uptake in Aspergillus.