TRANSPORT CHANGES RAPIDLY INITIATED BY SERUM ADDITION TO “CONTACT INHIBITED” 3T3 CELLS*

AUTOR(ES)

Cunningham, Dennis D.

RESUMO

Two- to fourfold increases in uridine and phosphate uptake were brought about within 10 to 15 minutes after adding fresh serum to confluent 3T3 cells. The stimulation of transport was specific, since no serum effect was observed for 3-O-methyl-D-glucose or amino acids. The early increase in RNA labeling, previously identified by 14C-uridine incorporation, could be accounted for by this transport effect. Increased labeling with 32P-phosphate of at least five phospholipids occurred soon after adding serum. This increase, however, could not be accounted for by increased transport. Both of these results suggest that specific early membrane changes are involved in “contact inhibition.”

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