Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm.

AUTOR(ES)

Trevors, J T

RESUMO

Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed with groundwater. The presence of wheat roots growing downward in the microcosms contributed only slightly to the movement of P. fluorescens C5t cells to lower soil regions of the loamy sand microcosms, but enhanced downward transport in the loam microcosms. Furthermore, the introduced P. fluorescens C5t cells were detected in the effluent water samples even after three flushes of groundwater and 10 days of incubation. As evidenced by a comparison of counts from immunofluorescence and selective plating, nonculturable C5t cells occurred in day 10 soil and percolated water samples, primarily of the loamy sand microcosms. Vertical soil microcosms that use water movement may be useful in studying the survival and transport of genetically engineered bacteria in soil under a variety of conditions prior to field testing.

Documentos Relacionados

• Growth of genetically engineered Pseudomonas aeruginosa and Pseudomonas putida in soil and rhizosphere.
• Microcosm for assessing survival of genetically engineered microorganisms in aquatic environments.
• Kelthane degradation by genetically engineered Pseudomonas aeruginosa BS827 in a soil ecosystem.
• Tracking genetically engineered bacteria: monoclonal antibodies against surface determinants of the soil bacterium Pseudomonas putida 2440.
• Pseudomonas fluorescens adhesion and transport through porous media are affected by lipopolysaccharide composition.