Transposon-induced inversion in Antirrhinum modifies nivea gene expression to give a novel flower color pattern under the control of cycloidearadialis.
AUTOR(ES)
Lister, C
RESUMO
The nivea (niv) gene of Antirrhinum majus encodes chalcone synthase, an enzyme involved in synthesis of anthocyanin pigments. The nivrec:98 allele contains a single copy of the transposon Tam3 inserted at the niv locus. A large chromosomal rearrangement derived from this mutant has been shown to be flanked by two copies of Tam3. In this study, we compared sequences involved in this rearrangement with their progenitor sequences and concluded that the rearrangement is an inversion resulting from an aberrant transposition occurring shortly after replication of Tam3 that left both copies of Tam3 active after the rearrangement. Excision of Tam3 from its position adjacent to the niv coding region resulted in a novel distribution of anthocyanin pigment in the flower tube, caused by the interaction of the new sequences with the remnant of the niv promoter. The new sequences upstream of niv serve both to enhance niv transcription and to redirect the pattern of gene expression, placing niv under the control of the gene cycloidearadialis, which determines the morphogenetic polarity of the flower.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=160384Documentos Relacionados
- Molecular Analysis of a Transposon-Induced Deletion of the Nivea Locus in Antirrhinum Majus
- Silencing of a gene adjacent to the breakpoint of a widespread Drosophila inversion by a transposon-induced antisense RNA
- Hybrid Dysgenesis-Induced Revertants of Insertions at the 5' End of the RUDIMENTARY Gene in DROSOPHILA MELANOGASTER: Transposon-Induced Control Mutations
- Molecular genetics of a transposon-induced dominant mutation in the Drosophila locus Glued.
- Transposon-induced promoter scrambling: a mechanism for the evolution of new alleles.