Transposon-induced promoter scrambling: a mechanism for the evolution of new alleles.
AUTOR(ES)
Kloeckener-Gruissem, B
RESUMO
We have studied a germinal revertant of the Mutator (Mu3)-induced mutation (Adh1-3F1124) of the maize alcohol dehydrogenase 1 gene (adh1). Transposon Mu3 was inserted at the TATA box of the promoter. The excision of Mu3 caused a complex, multibreakpoint DNA rearrangement with deletion, inverted duplication, and inversions affecting 430 nucleotides in the promoter region. These changes led to an unusual pattern of adh1 gene expression: increased levels of enzyme activity in one organ, decreased levels in another, and almost unchanged levels in a third organ. The evolutionary impact of transposon-induced promoter scrambling on generation of allelic diversity is discussed. We present a fragmentation model to help explain how transposon excision could induce multiple breakpoint aberrations without involving a homologous chromosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=42377Documentos Relacionados
- Molecular genetics of a transposon-induced dominant mutation in the Drosophila locus Glued.
- Molecular Analysis of a Transposon-Induced Deletion of the Nivea Locus in Antirrhinum Majus
- Isolation and characterization of a transposon-induced cytotoxin-deficient mutant of Pseudomonas aeruginosa.
- The Cloning of the Bar Region and the B Breakpoint in Drosophila Melanogaster: Evidence for a Transposon-Induced Rearrangement
- P Transposon-Induced Dominant Enhancer Mutations of Position-Effect Variegation in Drosophila Melanogaster